MOLECULAR DETECTION OF HUMAN PAPILLOMAVIRUS DNA IN SEROPOSITIVE SAMPLES USING PCR IN KOGI EAST, KOGI STATE, NIGERIA.

Abstract

Human Papilloma virus (HPV) is a double stranded (ds) DNA virus which encodes proteins and utilizes host cell metabolism and the immune system. Detection of human papillomavirus (HPV) genotypes is the base for prophylactic strategies against diseases and vaccination. This study was carried out to detect the prevalence of human papillomavirus genotypes circulating among the study population. A total of 195 cervical and serum samples were each collected by simple random sampling technique from three major hospitals in the zone. The IgM antibodies samples were subjected to molecular analysis (PCR). From the six seropositive samples, Human papilloma virus type16 was detected in 16.7% (1/6) of the samples that were positive for IgM antibodies to HPV while the rest showed negative. This indicates the circulation of other genotypes other than targeted genotypes.

Key words: Papillomavirus, genotype, infection and antibodies.