Phytochemical screening, in vitro antioxidant activity, and acute toxicity of methanolic leaves extract from Anisopus mannii. The

Abstract

This study investigated the phytochemical composition, the in vitro antioxidant activity, and acute toxicity of methanolic leaves extract from Anisopus mannii. The leaves were extracted using methanol in a Soxhlet apparatus, yielding 1.59% w/w of extract. The Qualitative Phytochemical screening confirmed the presence of alkaloids, flavonoids, tannins, saponins, terpenoids, saponins and phenolic compounds. The quantitative screening revealed alkaloids (4.50%), flavonoids (3.69%), saponins (3.26%), phenols (2.50%), terpenoids (1.86%), and tannins (1.56%).  Antioxidant potential was assessed using the DPPH radical scavenging method. The extract demonstrated concentration-dependent DPPH radical scavenging activity, with maximum inhibition of 77.75% at 60 mg/ml compared to ascorbic acid's 94.75%.  The calculated IC₅₀ value was within the range considered biologically active and showed a reasonable comparison with that of the standard (ascorbic acid). Furthermore, the acute toxicity assessment revealed no observable signs of toxicity or mortality in rats administered up to 5000 mg/kg, suggesting the extract is relatively safe at high doses. These findings contribute to the growing body of evidence supporting the medicinal use of Anisopus mannii and highlight its promise for future pharmacological applications.

Keywords: Anisopus mannii; phytochemicals; In vitro; antioxidant activity; DPPH assay; IC_50; acute toxicity; safety