Invitro anticoagulant potential of COMBINED Allium sativum bulb and Hibiscus sabdariffa leaves extract using different solvent.

Abstract

Cardiovascular diseases such as coronary heart disease and stroke are the top killers of people globally, and by 2030 almost 23.6 million people are projected to die from cardiovascular diseases. This study aimed to investigate the anticoagulant potential of combined Allium sativum bulb and Hibiscus sabdariffa using different solvent extracts (Methanol, Hexane,Ethylacetate and  Butanol ). Prothrombin time (PT) and Activated partial thromboplastin time (APTT) were accessed using different concentration of the combined extract (50mg/ml ,75mg/ml and 100mg/ml ) together with positive control (Enoxaheparin) and normal control. There is high significance differences that exist among the combined extract on different solvent (P<0.05)  Ethylacetate  was found to have have high prolonged PT and APTT followed by methanol , butanol and finally hexane. The ethylacetate having the highest prolonged PT and APTT could be due to being it semi polar solvent it can extract phytochemicals like alkaloids , flavonoids and phenols which are known to have anticoagulant property by inhibiting synthesis of thromboxane A2 induced by adeninediphosphate, arachidonicacid and collagen. This combined extract has demonstrated potential for use as an anticoagulant for preventing cardiovascular diseases and bleeding disorders.

Keywords: Anticoagulant activity, cardiovascular diseases, combined Garlic and Roselle,