EFFECTS OF MILLET BOUND POLYPHENOLS ON BLOOD AND AMNIOTIC FLUID GLUCOSE LEVELS IN PREGESTATIONAL HYPERGLYCEAMIC WISTAR RATS.

Abstract

During intrauterine life, hyperglyceamia has been shown to cause alterations of metabolic signaling. The aim of this study was to evaluate the effects of Millet Bound Polyphenols (MBP) on glucose levels (Blood and amniotic fluid) in pregestational hyperglyceamic Wistar rats. 30 female rats were divided into six groups of five animals each (n=5). Hyperglyceamia was induces using high fat diet and high fructose for six weeks, Glucose levels were monitored using a glucometer using the glucose oxidase principle, animals were then mated for induction of pregnancy. Group I served as control/normal control, Group II served as the negative control/ hyperglyceamic, Group III, IV and V were given 100mg/kg, 200mg/kg and 400mg/kg Millet Bound Polyphenols respectively. All administrations were orally given. Amniotic fluid glucose levels were determined using the glucose oxidase method after 9 days of MBP administration. The result obtained from the study showed that: Blood and amniotic fluid glucose levels were decreased in all pregestational hyperglyceamic groups treated with millet bound polyphenols across all the three groups compared to the negative control group after 9 days administration across all the three groups compared to the negative control group. It is therefore concluded that MBP has antihyperglyceamic property.Key words: Hyperglyceamia, Amniotic fluid, Blood, Glucose, MBP